Kinesin switch in neurites

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Kinesin switch in neurites

B efore axon specifi cation, seemingly identical neurites are not so alike after all. New research from Catherine Jacobson, Bruce Schnapp, and Gary Banker (Oregon Heath & Science Center, Portland, OR) reveals a hitherto unseen biochemical distinction in neurites of developing neurons. The distinction was noted while visualizing a constitutive version of the Kinesin-1 motor, which in mature neur...

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Cayman ataxia protein caytaxin is transported by kinesin along neurites through binding to kinesin light chains.

Deficiency of caytaxin results in hereditary ataxia or dystonia in humans, mice and rats. Our yeast two-hybrid screen identified kinesin light chains (KLCs) as caytaxin-binding proteins. The tetratricopeptide-repeat region of KLC1 recognizes the ELEWED sequence (amino acids 115-120) of caytaxin. This motif is conserved among BNIP-2 family members and other KLC-interacting kinesin cargo proteins...

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The structural switch of nucleotide-free kinesin

Kinesin-1 is an ATP-dependent motor protein that moves towards microtubules (+)-ends. Whereas structures of isolated ADP-kinesin and of complexes with tubulin of apo-kinesin and of ATP-like-kinesin are available, structural data on apo-kinesin-1 in the absence of tubulin are still missing, leaving the role of nucleotide release in the structural cycle unsettled. Here, we identified mutations in...

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Differential roles of kinesin and dynein in translocation of neurofilaments into axonal neurites.

Neurofilament (NF) subunits translocate within axons as short NFs, non-filamentous punctate structures ('puncta') and diffuse material that might comprise individual subunits and/or oligomers. Transport of NFs into and along axons is mediated by the microtubule (MT) motor proteins kinesin and dynein. Despite being characterized as a retrograde motor, dynein nevertheless participates in anterogr...

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Kinesin: switch I & II and the motor mechanism.

New crystal structures of the kinesin motors differ from previously described motor-ADP atomic models, showing striking changes both in the switch I region near the nucleotide-binding cleft and in the switch II or 'relay' helix at the filament-binding face of the motor. The switch I region, present as a short helix flanked by two loops in previous motor-ADP structures, rearranges into a pseudo-...

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ژورنال

عنوان ژورنال: Journal of Cell Biology

سال: 2006

ISSN: 1540-8140,0021-9525

DOI: 10.1083/jcb.1731rr3